Biocontrol of pathogenic salmonellain pleed and deveined shrimp using bacteriophages (Record no. 12135)
| 000 -LEADER | |
|---|---|
| fixed length control field | 03080nam a22001457a 4500 |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Sutapa Biswas |
| 245 ## - TITLE STATEMENT | |
| Title | Biocontrol of pathogenic salmonellain pleed and deveined shrimp using bacteriophages |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication | Panangad |
| Name of publisher | KUFOS |
| Year of publication | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 75 |
| Other physical details | HB |
| Accompanying material | CD |
| 500 ## - GENERAL NOTE | |
| General note | MFSc Thesis |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | ABSTRACT<br/>The use of lytic bacteriophages for the biocontrol of food borne pathogens in food<br/>industry is gaining increasing acceptance. In recent years it has become widely recognized<br/>that bacteriophages have several potential applications in the food industry as a biocontrol<br/>agent. But in the seafood industry we are still using chemicals to control the food borne<br/>bacterial pathogens. Hence this study has been under taken to control food borne<br/>Salmonella infections in peeled and deveined (PD) shrimp using bacteriophage.<br/>In this study we could isolate Salmonella phage from raw sewage sample collected<br/>from Fort Kochi using Salmonella Typhimurium (MTCC 1264) as the host bacterium. The<br/>host specificity of the isolated phage was determined by spotting the higher dilution of<br/>phage onto the bacterial overlay. The phage isolated in the present study showed high<br/>specificity towards the Salmonella Typhimurium whereas it didn’t show any specificity to<br/>the other isolates tested. The efficacy of the isolated Salmonella phage as a biocontrol<br/>agent against Salmonella was checked by conducting challenge experiments in PD shrimp.<br/>PD shrimp was experimentally contaminated with Salmonella Typhimurium (103 cfu/ml)<br/>and treated with Salmonella phage at a concentration of 7x108 pfu/ml and kept at 8°C with<br/>negative and positive controls which was mock treated with 2ml distilled water and 2 ml of<br/>freshly prepared Salmonella Typhimurium culture, respectively. The samples were<br/>collected from all the controls and treatments at four time intervals viz.0th, 3rd, 6th, 9th h.<br/>The results showed that the log reduction between the positive and treatment trials<br/>at 0th and 3rd hour interval as 0.24 and 0.95 log unit, respectively. But from 6th hour<br/>onwards the host bacterial count showed high reduction in Salmonella count when<br/>compared to 0th and 3rd hour. At the 6th hour, the Salmonella count obtained for the positive<br/>control was 3.08×103 cfu/ml and for the treatment, the bacterial count was 1.4×101. The<br/>log reduction in host bacterial count between the positive and treatment at 6th hour was<br/>2.34 whereas at 9th hour the log reduction was 2. 51 log unit. The statistical analysis using<br/>ANOVA showed that there was a significant difference in the bacterial count between the<br/>treatment and positive control as well as at various time intervals with 95 percentage<br/>confidence interval. Overall, the result indicates that the isolated phage is useful to control<br/>food borne pathogen Salmonella Typhimurium in PD shrimp. Hence the study shows the<br/>potential effectiveness of this isolated Salmonella phage as a biocontrol agent of<br/>Salmonella Typhimurium in PD shrimp. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Thesis |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | D-SpaceDigital Library MEENAM |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Books |
| Withdrawn status | Lost status | Collection code | Home library | Current library | Shelving location | Date acquired | Source of acquisition | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Reference | KUFOS Central Library | KUFOS Central Library | Thesis Shelf | 07/09/2016 | KUFOS | TH147 | Thesis |