Basic methods in protein purification and analysis (Record no. 1881)
| 000 -LEADER | |
|---|---|
| fixed length control field | 02874cam a22002894a 4500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| ISBN | 9780879698683 (hardcover : alk. paper) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| ISBN | 9780879698676 (pbk. : alk. paper) |
| 082 00 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.607 8 |
| Item number | SIM/BA |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Simpson, Richard J. |
| 245 00 - TITLE STATEMENT | |
| Title | Basic methods in protein purification and analysis |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication | Cold Spring Harbor, N.Y. : |
| Name of publisher | Cold Spring Harbor Laboratory Press, |
| Year of publication | c2009. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | vii, 436 p. : |
| Other physical details | ill. ; |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks. |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Proteins |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Proteins |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Proteins |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Chromatography |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Electrophoresis |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Immunoprecipitation |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Proteins |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Simpson, Richard J. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Adams, Peter D. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Golemis, Erica. |
| 856 41 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://www.loc.gov/catdir/toc/fy0903/2008031690.html |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Books |
| Withdrawn status | Lost status | Collection code | Home library | Current library | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Non-fiction | KUFOS Central Library | KUFOS Central Library | General Stacks | 04/05/2016 | 572.607 8 SIM/BA | 18061 | Books |