Comparison of Loop Mediated Isothermal Amplification(LAMP) Method With Conventional Polymerase Chain Reaction (PCR) for Detection of White spot Syndrome Virus (WSSV) in Field samples (Record no. 25110)
| 000 -LEADER | |
|---|---|
| fixed length control field | 01170nam a22001217a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 639.3 |
| Item number | DIV/CO |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Divyamol .K.K |
| 245 ## - TITLE STATEMENT | |
| Title | Comparison of Loop Mediated Isothermal Amplification(LAMP) Method With Conventional Polymerase Chain Reaction (PCR) for Detection of White spot Syndrome Virus (WSSV) in Field samples |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication | Panangad |
| Name of publisher | Dept .of Aquatic Animal Health Management |
| Year of publication | 2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 48p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | Abstract- LAMP is an efficient, specific, sensitive, and rapid strand displacement technique used for disease diagnosis in aquaculture. A highly specific LAMP procedure is standardized and optimized for WSSV. Based on the sequence of the ORF 14/15 region of Indian WSSV strains (Gen Bank accession no EF 468498 and EF 468499) six set of LAMP primers were designed. Time for the reaction was optimized for 30 min at an optimum temperature of 65°C. The sensitivity of this method is almost similar to that of nested PCR reaction. LAMP is cost effective when compared to PCR, therefore it is affordable for farmers. Rapidity, specificity and sensitivity of this technique make it an ideal diagnostic tool for detection of WSSV |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Withdrawn status | Lost status | Collection code | Home library | Current library | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Reference | KUFOS Central Library | KUFOS Central Library | Thesis Shelf | 14/08/2017 | 639.3 DIV/CO | TH162 | Thesis |