Phenotypic and Molecular Characterization of Isolated from Indian Oil Sardine Marketed in Cochin (Record no. 28259)
| 000 -LEADER | |
|---|---|
| fixed length control field | 02439nam a22001217a 4500 |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 664.94 |
| Item number | AHA/PH |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Ahana Vijayan |
| 245 ## - TITLE STATEMENT | |
| Title | Phenotypic and Molecular Characterization of Isolated from Indian Oil Sardine Marketed in Cochin |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication | Panagad |
| Name of publisher | KUFOS |
| Year of publication | 2020 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 97 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | ABSTRACT<br/>Fish are commonly marketed in chilled condition under aerobic storage.<br/>Pseudomonas spp. and Shewanella putrefaciens are the major microbial flora seen<br/>under aerobic ice storage. In this study phenotypic and genotypic characterization of<br/>P. aeruginosa was done considering its role as an opportunistic pathogen and its<br/>ability to cause acute and chronic infections which are life threatening. Samples were<br/>collected from 3 major markets in Cochin within a period of 8 months. A total of 48<br/>unique isolates were obtained, which were then subjected to a series of biochemical<br/>tests for the confirmation of the genus Pseudomonas. Thirty six isolates were<br/>presumptively identified as Pseudomonas sp. of which 9 were further confirmed as P.<br/>aeruginosa by 16SrRNA PCR. All these 9 P. aeruginosa isolates were then checked<br/>for the presence of 7 major virulent genes namely, oprI, oprL, lasB, toxA, exoS, nan1<br/>and phz by PCR using specific primers. All the isolates were found to be positive for<br/>oprI and oprL genes which are unique factors used for the rapid identification of this<br/>species. Further, lasB gene was detected in all the 9 isolates, whereas exoS gene was<br/>detected only in 6 isolates. None of these isolates were found to be positive for toxA,<br/>nan1 and phz by PCR. Antibiogram analysis was performed against a set of 18<br/>clinically used antibiotics. All were found to be sensitive to gentamycin, tobramycin,<br/>amikacin, aztreonam, ciprofloxacin, doripenem, imipenem, meropenem, norfloxacin,<br/>colistin and polymyxin B, but showed intermediate status to pipperacillin tazobactum.<br/>Only one isolate named as PA05 carrying lasB gene, was found to be resistant to 4<br/>antibiotics namely, ceftazidime, ciprofloxacin, norfloxacin and ofloxacin and MAR<br/>index was calculated as 0.22. Altogether, the study demonstrated the prevalence of P.<br/>aeruginosa in common seafood consumed domestically in Kochi and provides basic<br/>information on the virulent and antibiotic resistance profile of this pathogen, one of<br/>the most challenging organisms involved in a variety of nosocomial infections of<br/>humans. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Withdrawn status | Lost status | Collection code | Home library | Current library | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Reference | KUFOS Central Library | KUFOS Central Library | Thesis Shelf | 16/03/2020 | 664.94 AHA/PH | TH282 | Thesis |