Basic methods in protein purification and analysis
Published by : Cold Spring Harbor Laboratory Press, (Cold Spring Harbor, N.Y. :) Physical details: vii, 436 p. : ill. ; ISBN:9780879698683 (hardcover : alk. paper); 9780879698676 (pbk. : alk. paper).Item type | Current library | Collection | Call number | Status | Date due | Barcode |
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Books | KUFOS Central Library General Stacks | Non-fiction | 572.607 8 SIM/BA (Browse shelf(Opens below)) | Available | 18061 |
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572.6 WAL/PR Proteins of Animal Cell Plasma Membranes Vol 1 | 572.6 WAL/PR Proteins Biochemistry and Biotechnology | 572.6 WAL/PR Proteins Biochemistry and Biotechnology | 572.607 8 SIM/BA Basic methods in protein purification and analysis | 572.633 MOR/OX Oxidative folding of peptides and proteins | 572.636 WIL/HO How proteins work | 572.65 DAV/AM Amino Acids, Peptides and Proteins, Vol.36 |
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks.
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.
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