Normal view MARC view ISBD view

Comparison of Loop Mediated Isothermal Amplification(LAMP) Method With Conventional Polymerase Chain Reaction (PCR) for Detection of White spot Syndrome Virus (WSSV) in Field samples

by Divyamol .K.K Published by : Dept .of Aquatic Animal Health Management (Panangad) Physical details: 48p. Year: 2017

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings ( 1 )
Title notes ( 1 )
Comments ( 0 )

Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
Thesis	KUFOS Central Library Thesis Shelf	Reference	639.3 DIV/CO (Browse shelf(Opens below))	Available		TH162

Browsing KUFOS Central Library shelves, Shelving location: Thesis Shelf, Collection: Reference Close shelf browser (Hides shelf browser)

Previous	No cover image available No cover image available	No cover image available No cover image available	No cover image available No cover image available	No cover image available No cover image available	No cover image available No cover image available	No cover image available No cover image available	No cover image available No cover image available	Next
Previous	639.3 DEV/ST Studies on Fishing Systems of Mahanadi River, Chhattisgarh	639.3 DHI/EF Effect of Partial Replacement of Fish Meal with ULVA LACTUCA and PADINA TETRASTROMATICA On Growth Performances of Etroplus Suratensis(Bloch) Fingerlings	639.3 DIV/AS Assessment of Health Status of Sasthamkotta Lake with Special Focus on Nitrogenous Compounds	639.3 DIV/CO Comparison of Loop Mediated Isothermal Amplification(LAMP) Method With Conventional Polymerase Chain Reaction (PCR) for Detection of White spot Syndrome Virus (WSSV) in Field samples	639.3 DIV/EF Effect of Graded Level of Phytic acid on Growth Performance, Digestive Enzyme and Antioxidant Enzyme Activity in Oreochromis niloticus Fingerlings	639.3 DIV/EV Evaluation of cashew nut processing waste (CPW) as a non-conventional feed ingredient in the diet of tilapia Oreochromis mossambicus	639.3 DIV/IS Isolation and Characterization of new Potential Probiotic Bacteria Based on Quorum Sensing System	Next

Abstract- LAMP is an efficient, specific, sensitive, and rapid strand displacement technique used for disease diagnosis in aquaculture. A highly specific LAMP procedure is standardized and optimized for WSSV. Based on the sequence of the ORF 14/15 region of Indian WSSV strains (Gen Bank accession no EF 468498 and EF 468499) six set of LAMP primers were designed. Time for the reaction was optimized for 30 min at an optimum temperature of 65°C. The sensitivity of this method is almost similar to that of nested PCR reaction. LAMP is cost effective when compared to PCR, therefore it is affordable for farmers. Rapidity, specificity and sensitivity of this technique make it an ideal diagnostic tool for detection of WSSV

There are no comments on this title.

to post a comment.